What is cultured bezoar in vitro?

Professor Cai from Tongji Medical College of Huazhong University of Science and Technology and other scientific and technological workers have successfully developed "in vitro cultivation of bezoar" and obtained 1993 national invention patent and 1997 national first-class Chinese medicine certificate. It was included in the "863" plan in 2002 and won the second prize of national technological invention in 2003. In September, 2003, the National Pharmacopoeia Committee organized academicians, Professor Gao, Professor Zhang Boli, Researcher Zhou, Director Yao, and other well-known domestic authoritative experts to make a special trip to Wuhan for on-site inspection and certification, and agreed that "in vitro culture of bezoar is mature in technology, stable in quality, safe and effective, and can be used equally with natural bezoar." June 5438+October 2004 10, in vitro cultured bezoar was officially approved by the State Food and Drug Administration of the United States, and used in the same amount as natural bezoar to replace natural bezoar in Chinese patent medicines, especially in acute and severe treatment drugs.

"In vitro culture of bezoar" is to simulate the formation principle and biochemical process of bovine gallstones in vitro by using modern bioengineering technology. After bacterial culture, under the action of various enzymes, the stone-forming antagonistic components in bile were removed to make Shi Niu bile, and then the stone core was formed under the promotion of zinc acetate. Because of electrostatic attraction and bridging, organic polymers are of high biological quality.

The test results of quality standards approved by the state show that its technical parameters, quality indicators, functions and indications are consistent with those of natural bezoar. Through drug trials and double-blind methods, more than 850 clinical trials were conducted in 7 hospitals in three provinces. The results show that the quality of in vitro cultured bezoar completely meets the requirements of China Pharmacopoeia, and its curative effect and performance are very close to or even better than natural bezoar. Its main pharmacological components are more stable than natural bezoar, so it is an ideal substitute for natural bezoar.