When ion exchange and gel chromatography are packed in the column, bubbles will appear. How to deal with nodules?

You can wet the chromatographic column with an appropriate amount of organic solvent. If the chromatographic column is small, stir it evenly with a glass rod to eliminate bubbles.

Whether it is a dry column or a wet column, chromatographic liquid should be added, and there should be no bubbles at first. It is necessary to continuously elute with eluent, and elute the column with prepared reagent, which can not only eliminate bubbles, but also make silica gel fully settle, so as to make the chromatographic effect better. After there is no bubble in the column, the sample is analyzed by chromatography with eluent.

If there are bubbles in the column or the tightness of each part is uneven (and there can be no faults), it will affect the seepage velocity and color uniformity. Removal means stirring with a glass rod to drive away bubbles.

Extended data:

Some cations in the aqueous solution enter the counter ion layer, while the cations originally in the counter ion layer enter the aqueous solution. This same-sex ion exchange between the counter-ion layer and the aqueous solution with normal concentration is called ion exchange.

Ion exchange mainly occurs between diffusion layer and normal aqueous solution. Because the surface of clay particles is usually negatively charged, ion exchange is mainly cation exchange, so it is also called cation exchange. Ion exchange strictly abides by the equivalence law, that is, the cation equivalent entering the counter-ion layer is equal to the cation equivalent displaced from the counter-ion layer.

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