An unsterilized natural fermented liquid food product is available, and an experimental protocol is designed to analyze the diversity of microbial populations in it

1. Isolation. The natural fermented liquid food is inoculated in a sterile environment on a medium that can support the growth of a variety of microorganisms, such as nutrient agar, and in the case of special bacteria, such as lactobacilli, it is necessary to isolate lactobacilli on the medium, and cultivate to obtain individual colonies;

2. Identification. Identification of single colonies obtained by isolation, can take a variety of methods, such as, traditional morphological observation, biochemical reaction methods, but also molecular biology methods, such as 16Sr DNA sequence comparison, to obtain the identification of the results of the strain species is the microbial population.