How long does it take to knockout in E. coli

Nowadays, the most efficient knockout technology is the Red recombination system to realize efficient recombination of mutant gene cassettes in the E. coli genome, and then use its own Xer recombination system to realize efficient deletion of selective markers after mutation of target genes. The most efficient knockdown technology is the Red recombination system, which realizes efficient recombination of the mutant gene cassette in the E. coli genome, and then uses its own Xer recombination system to realize efficient deletion of selective markers after the target gene mutation.