Precautions for primary culture

I. Pay attention to contamination

1. Strictly carry out animal skin disinfection and use three sets of instruments to take materials. The skin of newborn animals was first sterilized with 2% iodine solution, and adult mice were first sterilized with 3%~5% iodine solution and then sterilized with 75% alcohol.

2. Strict aseptic operation was carried out to prevent bacterial, mold and mycoplasma contamination and avoid chemical contamination.

3, before sucking the liquid, the bottle mouth and pipette for flame sterilization; sucking the liquid, avoid the bottle mouth and pipette collision.

4, centrifugal tube into the table before, the mouth and wall of the tube should be sterilized.

5. When the experimenter leaves the ultra-clean bench, the working window should be closed with the elbow at any time.

6, used instruments with alcohol cotton balls to wipe away blood stains, move into another vessel to continue to disinfect, in the soaking instrument scissors mouth to fork open, tweezers elbow to put down, and cover disinfection.

Two, the use of equipment should pay attention to both flame sterilization, but also to prevent scalding, scalding cells. Pipettes sterilized by flame must be cooled with Hanks liquid.

Third, the temperature and humidity inside the ultra-clean table are large, and the heat dissipation inside the table is even slower when working in summer, so when mixing and inoculating cell suspensions, it is necessary to be a little farther away from the flame.