How to obtain a pure culture of a certain bacteria (or virus)? Briefly describe the whole process.

After the secretion is cultured for the first time, a circle is enough to pick out suspicious colonies, and then it is inoculated into a new medium for re-cultivation. This is the process of bacteria separation and purification, that is, pure culture. In the whole process, we must pay attention to aseptic operation, and when choosing live colonies, we must pay attention to distinguish them from miscellaneous bacteria.

Many bacteria cannot be cultured. Culturable bacteria need to check their culture medium formula and culture conditions. Viruses generally exist in the form of binary cultures, that is, susceptible bacteria. Of course, there are many that cannot be cultivated.

Extended data:

The most important thing in pure culture is the physiological study of microorganisms. Based on sterilization and separation, this method was established by L. Pasteur and R. robert R.Koch. In nature, some culture conditions are very difficult, especially those with close biological relationship and parasitic nutrition. There are also some organisms that are theoretically impossible to be cultured purely.

Baidu Encyclopedia-Microbial Pure Culture