Traditional Culture Encyclopedia - Traditional culture - How to isolate bacteria from soil
How to isolate bacteria from soil
The first step is to wash the soil with saline, then centrifuge it at a low speed to remove the upper layer of liquid
Then you have to identify the species you need, design or find a culture medium and sterilize it after preparation
The bacterial liquid is diluted to a certain proportion and then spread or streaked onto a plate lined with the medium or connected to the liquid medium at a certain proportion. What form of medium depends on the bacteria you need to isolate
Placed with the incubator for a number of hours
The above is the initial screening, the following needs to be further re-screening,
Pick out the colonies you need, and then improve the medium, and repeat the above steps
Until completely screened out of the target colonies
The above is a program that I came up with, I personally I happen to be a microbiology major, I personally believe that the preparation work before doing it should be done well, pay attention to more literature, more understanding of the characteristics of the target strains, using the fastest method
Wish you success
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