What are the methods of bacterial isolation and culture?

What are the methods of bacterial isolation and culture?

If it is a liquid medium, it is more difficult to separate. It needs special freeze-drying equipment and a lot of soft protective substances.

Therefore, in general, the best way is to inoculate bacteria on solid culture medium, so that the grown colonies or fungus moss are the bacteria you want to isolate.

Principle: By scribing on the plate, the mixed bacteria are fully dispersed on the surface of agar plate, so that a single bacteria can grow and reproduce at one point, forming a single colony, thus achieving the purpose of separating pure strains. If it is necessary to obtain purebred from the plate, select a single colony for purebred culture.