What are the commonly used fluorescent dyes in real-time fluorescent PCR?

Fluorescent dyes include saturated fluorescent dyes and unsaturated fluorescent dyes, and the typical representative of unsaturated fluorescent dyes is SYBR Green ⅰ, which is most commonly used now. Saturated fluorescent dyes include EvaGreen and LC Green.

SYBR Green I is the most commonly used DNA-binding dye in fluorescence quantitative PCR, which binds non-specifically to double-stranded DNA. In the free state, SYBR Green I emits weak fluorescence, but once it binds to double-stranded DNA, its fluorescence increases by 1000 times. Therefore, the total fluorescence signal emitted by a reaction is directly proportional to the outgoing amount of double-stranded DNA, and will increase with the increase of amplified products.