Experimental results of drug resistance of traditional bacteria

1. test error: even under strict experimental conditions, there may still be some errors due to technical or environmental factors. For example, the quality of paper, the cleanliness of laboratory, temperature and humidity may all affect the experimental results in paper-based drug sensitivity test;

2. Antibiotic resistance: If individual colonies grow in the inhibition zone, it may be because these strains are resistant to antibiotics. Some bacteria may develop resistance to antibiotics by producing specific enzymes or changing the permeability of cell membranes.

3. Stability of antibiotics: Some antibiotics may be unstable and easily damaged after contact with strains, thus reducing their antibacterial effect. This may also lead to the growth of individual colonies in the bacteriostatic circle;

4. Diversity of strains: In the drug sensitivity test, if the strains used are diverse, there may be different reaction results. Different strains may have different sensitivities to the same antibiotic.