Traditional methods for detecting salmonella

Gram staining was performed on the target bacteria. If it is Gram-negative bacilli, the oxidase and glucose oxidative fermentation (O/F) test is carried out. If oxidase is negative and glucose is fermented, it can be considered as Enterobacteriaceae. In addition, according to gluconate and phenylalanine deaminase tests, Enterobacteriaceae bacteria can be divided into three categories. Then, according to the color and morphology of the bacteria on the selective medium, the reaction on the kjeldahl disaccharide medium, the results of kinetics, indole, urease test (MIU) and IMViC test, the strain was comprehensively analyzed and identified. In Enterobacteriaceae, EPEC, Shigella and Salmonella should be grouped and typed by serological tests in addition to their biochemical reactions. For example, a bacterium grows as a red colony on MacKay plate and SS plate, but it is a gray, moist, medium-sized colony on blood plate, and Gram staining is negative. On KIA, acid and gas, such as H2S(-), MIU (++) and IMViC (++), are produced on the slope and bottom, which can be identified as Escherichia coli through the above biochemical reactions. The strain originated from diarrhea feces of 8-month-old infants, so the red colony on MAC agglutinated with EPEC antiserum, and the result was positive with EPEC multivalent antiserum, and then H antigen was determined. The appraisal result is EPECO 1 19:H6, and the final appraisal report can be issued at this time.