Diagnostic methods of herpes simplex virus

Susceptible cells were inoculated with saliva, spinal fluid, oral cavity, cervical secretion, vaginal secretion or curettage of cornea and conjunctiva, and cultured for 1 ~ 2 days. The cells swelled, rounded and fused with each other, which can be used for preliminary diagnosis. Then HSV was identified by immunofluorescence (IFA) and enzyme-linked immunosorbent assay (ELISA). If necessary, type.

(2) antigen detection

Direct detection of antigens in cells or secretions by IFA and ELISA can quickly diagnose HSV infection.

(III) Antibody detection

Detection of antibodies in patients' serum by complement fixation test and ELISA can be used to diagnose primary infection, but it can not be distinguished from recurrent infection. Because of the high infection rate of HSV, widespread latent infection and high antibody level in serum, it is difficult to observe the increase of antibody titer during repeated infection. Detection of antibodies in spinal fluid is of great significance to HSV infection in nervous system.

In addition, DNA molecular hybridization and PCR also show great advantages in detecting HSVDNA. Now it is mostly used in experimental research and will be popularized and applied in clinic.