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Cell Culture Essay Sample

Cell culture technology is the basis of cell biology research and occupies a very important position in the field of biotechnology research. The following is a cell culture paper that I have organized for you for your reference.

Cell culture thesis a

Initial exploration of the teaching reform of the cell engineering course

Abstract of cell culture thesis

Abstract Cell engineering is a professional compulsory course for biotechnology majors in China's undergraduate colleges and universities. In view of the characteristics of the course, this paper carries out active exploration from optimizing theoretical teaching and strengthening practical teaching in order to provide reference for the teaching reform of cell engineering course.

Content of Cell Culture Paper

Keywords Biotechnology, Cell Engineering, Teaching Reform

CCTI: G424 Literature ID: A

Discussion on Teaching Reform of Cell Engineering Course

LI Anzheng

(Teaching and Research Section of Biotechnology, Hubei University of Chinese Medicine, Wuhan, Hubei 430065)

Abstract Cell engineering is a professional required course for undergraduate biotechnology major of universities and colleges in china. In this paper In this paper, according to the characteristics of this course, positive exploration was carried on to optimize the theory teaching and strengthen the practice In this paper, according to the characteristics of this course, positive exploration was carried on to optimize the theory teaching and strengthen the practice teaching, which may provide references to teaching reform of cell engineering course.

Key words biotechnology; cell engineering; teaching reform

The 21st century is the century of life (biological) science. Biotechnology is a comprehensive technology system that applies the research results of life sciences to transform and utilize living things or components of living things, including cell engineering, genetic engineering, enzyme engineering, fermentation engineering and biochemical engineering. Among them, cell engineering is the application of the use of biological research accumulated knowledge and technology, the development and utilization of biomaterials or biological systems at the cellular level, and a certain process to obtain products (cell lines, cell lines, organisms or their secondary metabolites) of the relevant theories and techniques of the discipline.

Our biotechnology program began enrolling in 2005, after eight years of professional construction, has now formed a more complete theoretical and practical teaching system. At present, cell engineering has become one of the main courses of biotechnology majors in colleges and universities. Learning this course well will provide a good theoretical and technical foundation for students to engage in relevant research in the field of biology and biotechnology industry related to cell engineering in the future. Implementing the teaching concept of "taking students as the main body", improving teaching quality and cultivating high-quality applied talents are the goals that many institutions, including ours, have been pursuing diligently. Combined with the characteristics of the cell engineering course and the actual situation of our school, the Biotechnology Teaching and Research Department has carried out a series of reforms and explorations on the teaching content, teaching methods and experimental teaching of the course.

1 Theoretical Teaching Reform

1.1 Optimization of Teaching Content

Teaching content determines the basic knowledge structure of the students, affecting the formation of the students' basic abilities, and whether or not the content of the teaching content is full of novelty has a significant impact on the improvement of teaching quality. In view of the fact that a high-quality course is a model course with first-class teaching team, first-class teaching content, first-class teaching methods, first-class teaching materials, first-class teaching management, etc., in the teaching of cell engineering, combined with the characteristics of traditional Chinese medicine in Chinese medicine colleges and universities as well as the reality of the faculty in the biotechnology teaching and research department, we have introduced the "211" national curriculum created by Huazhong Agricultural University, which is a national high school. We introduced the content of cell engineering, which is a national high-quality course created by Huazhong Agricultural University, a university in China.

In the selection of teaching materials, the book "Plant Cell Engineering", edited by Prof. Jun Liu, published by Higher Education Publishing House, is used as a teaching reference book, which systematically introduces the technology of plant tissue and cell culture, not only explains the operation method, but also discusses the theoretical principles, which is more suitable for undergraduate study.

At the same time to improve the syllabus, the teaching content of the appropriate increase or decrease. Cell engineering is closely related to other biological sciences, such as plant biology, animal biology, cell biology and molecular biology as a prerequisite course, so some chapters of the content will be repeated, which can be briefly mentioned or questioned to show the review. Such as plant endosperm culture involves endosperm development in three ways (nuclear endosperm, cellular endosperm and bog-type endosperm), which has been studied in plant biology, can be skipped here.

In addition, the new century, including cell engineering, biotechnology, including the new achievements in the field of rapidly changing, so the teacher, not only to teach students the basic principles of the field, the basic methods and basic technology, but also to the latest research progress into the teaching. This requires that teachers must be able to keep abreast of the latest developments in the field (consult the authoritative literature at home and abroad), and timely add the relevant content to the teaching content, so as to stimulate the students' interest in learning, and enhance the students' self-consciousness and creativity in learning. Requirements for careful and meticulous preparation for each and every class (including laboratory classes), and teaching reflection after class, so although the course is basically repeated every year, but every year there is a new experience, you need to spend a lot of time to carefully prepare for the class.

1.2 Reform of teaching methods

Stimulate students' interest in learning, and give play to their subjective initiative. The so-called interest is the best teacher, with interest in learning, can play the students' subjective initiative, the teaching effect is undoubtedly greatly beneficial. University of traditional teaching mode is often indoctrination, the teacher is the transmitter of knowledge; new teaching modes such as heuristic teaching requires the teacher from the transmitter of knowledge into the learning of the guidance of the inspirer, stimulate students' interest in learning, mobilize students to learn the initiative and enthusiasm. Therefore, in the teaching process, you can choose a chapter or a knowledge point of cell engineering content, try to let the students experience classroom teaching, in order to cultivate the students' independent thinking ability, the ability to review the literature, the ability to summarize the ability to summarize and the ability to express their language.

Focus on teacher-student interaction, actively guide students to learn. Generally, a few minutes will be spent on reviewing the old class before the new class, that is, the content of the last class to put forward a few questions for students to think about the answer. This can not only consolidate what has been learned, but also connect the content of the new class, can be said to carry on the next, two in one. In addition, in the process of teaching also need to observe the students on a certain knowledge mastery, especially involving some of the content of the prerequisite courses, but also at any time to ask questions and do the answer. All the questions, to take into account every student, that is, every student has the opportunity to answer the question; the students who answer well should be strongly praised, the students who do not answer up should also be encouraged.　Carefully produce multimedia courseware, optimize multimedia teaching. With the improvement of social and economic level and the progress of science and technology level, make full use of multimedia and other modern teaching means is also the inevitable requirements of full-time teachers. At present, most of the classrooms in our school are equipped with multimedia teaching system, and the course of cell engineering also adopts multimedia lectures. How to realize the organic combination of the outline of traditional board teaching and the large amount of information of multimedia teaching is a problem that needs to be thought about in the process of teaching. In the production process of multimedia courseware (PPT slides), adhere to the principle of "fewer words but no shortage of charts and graphs, but not miscellaneous", will be the main points of the traditional board concentrated in one of the slides, and then add a number of hyperlinks with a chart for each point for detailed description, to achieve both to play the advantages of multimedia teaching, but also Without losing the effect of traditional teaching. At the same time, the multimedia courseware can also improve the foreign language (English) professional vocabulary, increasing the students' professional English foundation.

2 Practical Teaching Reform

Cellular engineering is a highly practical subject. In the experimental content setting, in line with the integration of teaching resources, optimize the teaching content of the principle of biotechnology professional training program in our school will include cell engineering, including several professional courses of the experiments to be integrated, the opening of a comprehensive experiments in biotechnology. Among them, the corresponding cell engineering part takes animal cell culture and plant tissue culture process as the basic content. In the experimental process, the former experiment prepares for the latter experiment, and the latter experiment is the depth of the former experiment. Students need to participate in the whole experimental process from the preparation of culture medium to the sterilization and inoculation of materials to the observation of experimental results. Considering the failure of an experiment due to contamination and other reasons, the instructor should pay attention to the culture situation and take preventive measures, such as preparing more experimental materials; or in addition to the demonstration of the experimental process, the instructor will also participate in the experiment as one of the experimental groups. The report of the experiment is written to require students to report the results of the experiment, even if the results of the failure is required to write and analyze the reasons.

3 Conclusion

Curriculum teaching is an important means of realizing the goal of education and training. In the teaching process of cellular engineering courses, we optimize the teaching content, the introduction of "211" universities and digest the fine courses, the latest scientific research results into the teaching content; the use of flexible and diverse teaching methods and multimedia teaching methods, so that the quality of teaching has been improved accordingly. In the future teaching process will keep pace with the times, constantly summarize experience, further improve the teaching content, teaching methods, especially to strengthen the opening of the cellular engineering experiments, enrich the experimental content, the cellular engineering courses to raise the level of teaching to a new level.

Cell Culture Essay Literature

[1] Liu Jun, Xie Conghua. Plant cell engineering (2nd edition) [M]. Beijing: Higher Education Press, 2011.

[2] Jiang ZH, Zhou DX. Exploration of cellular engineering teaching reform in local undergraduate colleges and universities [J]. Education Teaching Forum, 2013(27):52-53.

[3] Wang Yi, Liu Siyan, Sun Chunyu et al. Thoughts and Practices on Cultivating Students' Scientific Research Ability in the Teaching of Plant Cell Engineering Course[J]. China Out-of-School Education (Theory), 2008(9): 85-86.

[4] YANG Qingling, ZHANG Yao, CHEN Changjie et al. Research on the construction of comprehensive experiments in biotechnology[J]. Journal of Bengbu Medical College, 2009(34):166-168.

Cell Culture Thesis II

Cell engineering teaching reform and exploration

Cell Culture Thesis Abstract

Abstract According to the actual teaching of cell engineering, from the faculty, teaching content, teaching methodology and assessment methods, etc., to discuss the reform of cell engineering.

Cell culture thesis content

Keywords cellular engineering; teaching reform; assessment methods

Cellular engineering is a highly integrated discipline combining theory and practice, is the application of the principles and methods of cellular biology and molecular biology, at the cellular level, research, transformation of the genetic material of life, in order to obtain the cell line or biological organisms with the target traits. It is the application of the principles and methods of cell biology and molecular biology, at the cellular level, to study and transform the genetic material of life, in order to obtain a cell line or organism with the purpose of the traits of the theory and technology of the discipline, which is not only an important part of the modern biotechnology, but also an important technological tool for modern biological research, and occupies an important position in the curriculum of the life sciences and related disciplines in colleges and universities [1-2]. Learning this course well will lay a good theoretical and technical foundation for the students to engage in relevant research in the field of biology and biotechnology industry related to cell engineering in the future. Teachers must give full play to their own professional advantages, adapt to the needs of the development of the discipline, actively guide students to scientific knowledge and interest in the field, and systematically grasp the relevant principles, methods, technologies and progress; at the same time, cultivate students' comprehensive ability to enhance the teaching effect. In the process of continuous exploration, combined with the characteristics of cellular engineering, to improve the quality of cellular engineering teaching.

1 Strengthen the construction of faculty

1.1 Form a teaching team

In order to solve the problem of large subject span and different backgrounds of the teaching content, break the traditional teaching mode of one person, form a teaching team, and divide the teaching content into different teaching modules. Each module is undertaken by teachers with the appropriate professional background, trying to keep up with the cutting edge of each teaching content.

1.2 Improve the teaching level of teachers

In order to improve the level of teaching, the members of the group regularly study and discuss modern teaching ideas and modern teaching methods; regularly organize the experts who have made great achievements in teaching methodology to listen to lectures and conduct targeted evaluation of lectures; supervise the teachers to prepare lectures carefully and strive for excellence in their business, and in particular, require that the content of the teaching should be closely followed by the forefront of the discipline to make themselves become real experts and scholars of their own specialty. Become the real experts and scholars of this specialty, standing in the forefront of the development of this professional knowledge. At the same time, from time to time to carry out self-assessment and mutual evaluation, in order to promote the improvement of the overall level of teaching.

2 integration of scientific and technological development, reform, update the teaching content

Cellular engineering is a wide range of comprehensive disciplines, whether it is the teacher's teaching, or student learning has a certain degree of difficulty, which requires the selection of the content of the lectures, that is, pay attention to the content of the systematic and complete, but also ensure that the content of the lectures of the comprehensive, interesting, practical. Combined with the teaching purpose of biotechnology and the existing teaching materials, the content of the course mainly focuses on the following aspects: firstly, the foundation of cell engineering, including the basic concepts, the main research content, the basic facilities, conditions, methods and techniques of cell culture, etc.; secondly, the plant cell engineering, including the plant tissue culture, detoxification and fast propagation, haploid induction and breeding, embryo culture, somatic embryogenesis and artificial seed technology, protoplast fusion, seed production, and the development and application of the cell culture technology, and the development of the plant culture system, which is a very important part of the course. Seed technology, protoplast fusion, chromosome engineering, transgenic technology, etc.; Third, animal cell engineering, including animal cell culture, cell fusion, chromosome engineering, cell recombination and cloning, transgenic animals and bioreactors, etc.; Fourth, the practice and application of cellular engineering, including cellular engineering and its related technologies, the current status of the development of the development of the application of the current situation, and its industrialization and prospects for development.

In addition, due to the rapid progress of science and technology in today's world, the amount of knowledge and information is growing rapidly, the content of cellular engineering research is also changing rapidly, and new technologies and methods are constantly emerging. This requires teachers in the teaching of timely adjustment and updating of the teaching content, standing in the forefront of modern scientific and technological development, grasp the latest developments in the field of bioengineering science and technology, in a timely manner, these developments, research results and major issues to be tackled into the content of the teaching, stimulate students to learn and explore new knowledge interest, improve students' overall ability. For example, when explaining "cloning technology", the latest domestic and international achievements are disseminated to students in a timely manner, including December 14, 2007, South Korea, Professor Kong a through an adult female Turkish Angora cat epidermal cells cloned three white kittens containing fluorescent proteins; in January 2009, Cloning and Stem Cells magazine reported in its online edition that the research results and major topics to be tackled are integrated into the teaching content, stimulate students' interest in learning and exploring new knowledge, and improve their comprehensive ability. Cloning and Stem Cells magazine online version reported that the Shandong Province Stem Cell Engineering Research Center, Yantai Yuhuanding Hospital successfully obtained human cell cloning embryos. Another example is the explanation of "chromosome engineering", combined with the latest results of the 2009 Nobel Prize in Physiology and Medicine.

3 Reform of teaching methods

3.1 Inspirational, inquiry-based teaching methods

As a comprehensive technical course, the chapters of cell engineering are not very logical, less theoretical expression, more details of the application of technology, to the lack of basic knowledge of the students in the understanding of the content of the textbook to bring a greater degree of difficulty in memorization. Therefore, in the teaching process, we should vigorously advocate the heuristic, problem solving, discussion, training and practice teaching methods, encourage students to boldly question, free to explore, maximize students' learning initiative, enthusiasm and creativity. For example, in the process of teaching cellular recombination and cloning, only explain the principles of cellular recombination and cloning, and the latest progress of cloning by the students in the information, to do the explanation and summarize, by the teacher and other students to give the evaluation and correction.

3.2 Application of modern teaching methods to improve the quality of teaching

Cellular engineering is a fundamental and practical subject, the course of the large amount of information, the content is basically microscopic level, the traditional mode of teaching can not be presented to the students of such a large amount of information content of the course, but also easily caused by the students on the course of the slack, reduce the interest in learning, affecting the effect of teaching. In order to improve the teaching effect, the computer as a tool, through multimedia, teaching video, network resources, CAI courseware and other modern teaching methods, not only can provide students with colorful teaching information, but also provide a more beautiful human-computer interaction interface, fully mobilize the students' emotions, feelings, attention and interest [3]. This can make those abstract, difficult to observe under ordinary conditions of the process of visualization and image display, is conducive to the growth of students independent and flexible analysis of the problem, problem-solving ability, improve the quality of teaching, while stimulating students' interest in learning.

3.3 Carry out a variety of teaching activities

In addition to normal lectures, but also try to break the conventional teaching methods, to carry out colorful teaching activities, for the cultivation of students' interest in learning, and fully mobilize students to learn the enthusiasm has a very good effect [4]. Such as in the middle of the term, to the students assigned to write a review of the task, the title and content of their own, as long as the students are interested in, and the content of the subject can be related. Students through the search for information, a strong interest in biology, and even have a desire to work in this area in the future, some also took the initiative to find the teacher, applying for early entry into the laboratory. Another example is in the teaching process, try to let the students after learning each chapter of the content of their own test questions and give answers, and then submitted in the form of homework, the teacher synthesized the topics prepared by the students and all aspects of the information to establish a question bank. This form, on the one hand, reflects the respect for students, trust students teaching principles, while greatly mobilizing students to learn cellular engineering enthusiasm and initiative; on the other hand, the process of student compilation is also the process of learning and mastery, so that students learn to achieve twice the result with half the effort.

4 Reform of the assessment method

At present, most college students are dissatisfied with the final exam "a test for life", therefore, the development of scientific and feasible assessment methods to improve students' motivation to learn and improve the effectiveness of teaching has an important role. In order to guide the overall development of students, scientific evaluation of student learning, the course for the existing problems in the examination, from the following aspects of the examination mode of reform exploration: First, in the examination content, in addition to including the basic theory of the course, the basic knowledge, basic skills, etc., while also examining students in the integration on the basis of analyzing and problem-solving ability; Second, to strengthen the examination of the usual results, the classroom attendance, answering questions and answers, the classroom attendance, answering questions and answers to the questionnaire, the examination of the classroom, the classroom attendance, answering questions and answers to the questionnaire. Secondly, to strengthen the assessment of regular grades, class attendance, answering questions, assignments, discussions and classroom narratives will be recorded in the regular grades; thirdly, the final total rating will be 30% of the regular grades (assignments, attendance, classroom participation, etc.), 10% of the regular classroom activities, innovative activities (including writing a research review, the use of cellular engineering technology, the design of solutions to solve real-world problems, the establishment of an interest group, participate in research, classroom Lecture, etc.), 60% of the final exam (focusing on the ability to use theoretical knowledge to solve practical problems).

5 Conclusion

In the situation of scientific and technological competition, competition for talents, the economy and the rapid development of science and technology, through the research and exploration of cellular engineering course teaching system, the latest scientific and technological developments into the teaching content, the use of advanced teaching content and modernized teaching methods and means, reform of the assessment method, the reform of the teaching of cellular engineering course has made some achievements. In the future, we will continue to learn to master new knowledge, improve our own theoretical cognitive level, at the same time, each time before the class carefully prepared, and in the teaching practice of the course teaching system to improve the quality of teaching cell engineering.

Cell Culture Literature

[1] __Yong. Cell engineering [M]. Beijing:Science Press,2003.

[2] HU Shanglian,SUN Shou,CAO Ying. Exploration and practice of theoretical and practical teaching system of plant cell engineering[J]. China Out-of-School Education:Theory,2008(2):136-137.

[3] Zhang Yichun. A Practical Tutorial on Modern Educational Technology [M]. Nanjing:Nanjing Normal University Press,2005.

[4] Liang Yilong,Wei Jinmin,Zhang Succession. Exploration of cell engineering teaching reform[J]. Laboratory Science,2009(4):25-26.