Advantages of traditional methods of antibody preparation

(1) The traditional method of antibody preparation is to repeatedly inject a certain antigen into the animal, so that B cells and memory cells proliferate and differentiate into plasma cells, which produce antibodies, and then isolate the required antibodies from the animal.

(2) Inducing animal cell fusion, the unique method is to use inactivated virus as an inducer, hybridoma cells are characterized by a large number of proliferation, but also to produce specific antibodies.

(3) a patient continuous every other week injection of a certain murine monoclonal antibody treatment of a viral infectious disease, the beginning of the viral load significantly reduced, after a period of time, this viral load began to rise, the reason for the rise may be a mutation of the virus, but also may be the human body to produce anti-mouse antibody antibody, neutralization of the murine antibody, so that the murine antibody can not play a role in the fight against the virus.

(4) The advantages of monoclonal antibodies are high specificity, high sensitivity and high yield. Protein engineering is needed to modify the structure of the mouse-derived antibody molecule, and the modified mouse-derived antibody molecule can reduce the human response to the mouse-derived antibody.

Therefore, the answers are:

(1) B cells and memory cells Serum

(2) Inactivated viruses can both proliferate in large numbers and produce specific antibodies

(3) Mutated antibodies

(4) Strong specificity, high sensitivity, and high yield protein engineering