Traditional Culture Encyclopedia - Traditional customs - Advantages and disadvantages of ultraviolet spectrophotometry
Advantages and disadvantages of ultraviolet spectrophotometry
Disadvantages: the accuracy is not high.
In actual measurement, the solvent is put into another equivalent absorption pool, and the transmission intensity is compared with the analyzed solution, that is, A = lg( I solvent /I solution) ≈ lg (I 0/ I) absorbance is added: A total λ = a1λ+A2λ+... The limitation of the application of λ Beer's law is that it is only applicable to dilute solution, chemical deviation and λ.
Extended data:
Spectroscopy is a method based on measuring the wavelength and intensity of emitted, absorbed or scattered radiation produced by the transition between quantized energy levels in matter when matter interacts with electromagnetic radiation. Spectroscopy can be divided into emission spectrometry, absorption spectrometry and scattering spectrometry; Or divided into atomic spectrum and molecular spectrum; Or divided into energy level spectrum, electron, vibration spectrum, rotation spectrum, electron spin spectrum and nuclear spin spectrum. ?
Baidu Encyclopedia-Ultraviolet-Visible Spectrophotometry
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