What are the methods of plant single cell culture

Plate culture method:

Features: single cells in the medium is evenly distributed, easy to spot observation, easy to screen; poor aeration, excreta are easy to accumulate resulting in cell toxicity.

Watchful culture

Features: simple, high success rate; can not be directly observed under the microscope.

Feeder layer culture

Features: easy to operate; cannot track cell division and cell mass formation under the microscope.

Microchamber culture method

Features: continuous microscopic observation during the culture process; squid medium is less, it is difficult to maintain water, the composition of the medium and pH, etc. are also dissolved in the change, the cells are often unable to grow again after short-term culture.

Liquid shallow static culture method

Features: easy to add fresh medium, can be microscopic.