How is the surface of disposable cell culture dish treated?

PS plastic surface itself is not hydrophilic, and cells cannot grow on it. Before it is used in cell culture, it needs hydrophilic surface treatment. Therefore, the surface adhesion (hydrophilic) treatment technology and technology of cell factory containers has become the key technology of cell factory.

At present, there are three hydrophilic treatment technologies for PS plastic surface: atmospheric pressure glow discharge technology, TC treatment technology and vacuum plasma RF discharge technology. According to advanced technology, they are called three-generation technology: the first generation: atmospheric pressure glow discharge;

This technology ionizes oxygen and nitrogen in the air by glow discharge, and then grafts to PS surface to form a charged surface, thus having the ability of cell adhesion.

Second generation: improved atmospheric pressure glow discharge (TC treatment technology);

Supplementing some ionizable gases (such as oxygen and ammonia). ) to generate more charges, thereby increasing the hydrophilicity of the culture container surface.

The third generation: vacuum plasma surface treatment technology;

One or more easily ionized high-purity gases are quantitatively added into a culture container in a vacuum chamber. Under high-voltage RF discharge, high-purity gas is ionized and grafted onto the surface of the vessel, forming strong hydrophilic (adhesion) performance.