Traditional Culture Encyclopedia - Traditional festivals - The process of preparing monoclonal antibodies in high school biology? What are the advantages of monoclonal antibodies?
The process of preparing monoclonal antibodies in high school biology? What are the advantages of monoclonal antibodies?
Extraction of b cells from specific immune experimental animals
B cells and myeloma cells were fused into hybridoma cells by animal cell fusion.
The hybridoma cells which can proliferate indefinitely and produce specific antibodies were screened by specific selective medium.
In vivo or in vitro culture. In vivo culture means injecting hybridoma cells into the abdominal cavity of experimental animals and extracting them from ascites in the future. In vitro culture, that is, using the method of animal cell culture, will be extracted from the culture solution in the future.
Monoclonal antibodies have the advantages of high specificity and sensitivity, and can be prepared in large quantities.
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