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Two-stage countercurrent extraction process of penicillin

1. Firstly, penicillin fermentation broth was mixed with inorganic salt solution and extractant to extract penicillin from fermentation broth.

2. Secondly, two-stage countercurrent extraction is carried out on the extraction liquid, and the extractant is continuously recovered.

3. Then the recovered extractant is mixed with penicillin, and the penicillin is separated by two-stage countercurrent extraction.

4. Finally, the refined penicillin is crystallized.