The function of qualitatively adding acetic acid to urine protein by heating acetic acid method is

Make the PH value of urine close to the isoelectric point of protein.

Heating acetic acid method, heating to denature protein, adding proper amount of acetic acid (about110 of urine volume) to make the pH value (pH value) close to that of protein (pH value 4.7), further precipitating protein, and eliminating the turbidity of phosphate and carbonate caused by heating.

Urine protein qualitative test is a meaningful examination method for diagnosis, treatment and curative effect observation of renal diseases. There are many methods, such as aminosalicylic acid method, acetic acid heating method, heating acetic acid method, sulfosalicylic acid method, dry chemical test method and so on. Here is a brief introduction: 1. Aminosalicylic acid method: Aminosalicylic acid can form insoluble protein salt precipitate with protein, and the amount of protein can be estimated according to the formation. This method is sensitive, so it should be noted that there are too many uric acid or urate in urine, and false positive may occur with iodine-containing contrast media. 2. Acetic acid heating method: protein coagulates and degenerates into white turbidity, and after heating, acid is added to eliminate the turbidity formed by phosphate, so that the accuracy is improved and the sensitivity is slightly lower. 3. Heating acetic acid method: protein is denatured by heating, and an appropriate amount of acetic acid (about the urine volume of110) is added to make the pH value (pH value) close to that of protein (pH value 4.7), so as to further precipitate protein and eliminate the turbidity of phosphate and carbonate produced by heating.