How to culture microorganisms?

Washing the bacteria from a solid medium of Nakai biotechnology and pouring it into a liquid medium, or from a liquid culture, using a pipette to pick up the bacteria solution into the liquid medium, or transferring the bacteria solution from a liquid culture to a solid medium can be referred to as liquid inoculation

Puncture inoculation

This method is often used in the preservation of anaerobic strains of microorganisms, or when studying microbial dynamics. This method is often used when preserving anaerobic species or studying the dynamics of microorganisms. The inoculation tool used to perform puncture inoculation is an inoculation needle. The medium used is usually semi-solid medium. Its practice is: dip the inoculation needle into a small amount of bacteria, along the center of the semi-solid medium to the bottom of the tube for a straight line puncture, such as a bacterium with a flagellum and can move, then in the puncture line around the ability to grow

Live inoculation

Live inoculation is specifically for the cultivation of viruses or other pathogenic microorganisms of a method, because the virus must be inoculated with live organisms in order to grow and reproduce. The living organism used can be a whole animal; it can be some isolated living tissue, such as a monkey kidney; or it can be a developing chicken embryo. Inoculation is by injection, can also be mixed feeding

Pouring mixed inoculation

The method is to be inoculated with microorganisms to be put into the medium of the sea organisms in the petri dish, and then poured into the solid medium cooled to 45 ° C or so, quickly and gently shaking, so that the bacterial liquid to achieve the purpose of dilution. After the plate is solidified, it can be incubated at a suitable temperature, and individual microbial colonies can be grown

Line inoculation

This is the most commonly used inoculation method. That is, in the solid medium surface for back and forth linear movement, can achieve the role of inoculation. Commonly used inoculation tools are inoculation ring, inoculation needle and so on. In the oblique inoculation and plate demarcation is commonly used in this method

Coating inoculation

with the pouring of mixed inoculation is slightly different, that is, first pour a good plate, let it solidify, and then pour the bacterial liquid into the plate above, and then quickly with the coating stick in the surface of the back and forth around the coating, so that the bacterial liquid is uniformly distributed, you can grow individual microbial colonies

This is the most commonly used method of inoculation.