Disadvantages of nucleic acid probe hybridization technology

Special laboratories that need to use radioisotopes have long exposure time for self-development.

Isotope-labeled probes are difficult to preserve, and radiation pollution is harmful to health, especially high-quality metaphase chromosome specimens are difficult to obtain on the basis of cell culture, and observation is time-consuming and interphase nuclear research is difficult.

In situ hybridization is characterized by hybridization on metaphase chromosome specimens on microscope slides.