Other related to matrix-assisted laser-resolved ionization time-of-flight mass spectrometry

It has been predicted like this : "This century will be the century of TOF and FT!"

Molecular weight is the most basic parameter of physical and chemical properties of organic compounds. The correct molecular weight often represents the correct structure of the organic compounds and biological macromolecules measured.MALDI-TOF is a soft ionization technique that produces no or few fragment ions. It can be applied directly to the analysis of mixtures and can also be used to detect the presence and molecular weight of impurities in a sample. Molecular weight is also the primary parameter in the identification of biomolecules such as peptides and proteins, and is one of the important data for the approval of genetically engineered products.

MALDI-TOF has an accuracy of up to 0.1%~0.01%, which is much higher than that of the SDS electrophoresis and high-performance gel chromatography techniques that are routinely used today, and can currently determine the molecular weight of biomolecules up to 600 KDa.