What are the common methods used in isolation and purification of microorganisms?

Dilution inverted plate method?

Plane marking method?

Single cell picking?

Use selective media separation?

Purification:?

1. If you don't know the characteristics of the microorganism you want to purify, and don't know its colony characteristics and morphological size at all, then according to the characteristics of the bacteria you want to isolate, find a suitable primary screening medium and find the bacteria you want to purify. For example, cellulose bacteria, you add cellulose powder or CMC-Na as carbon source to the culture medium, so that you can screen out cellulose-decomposing bacteria. Then continue the purification in the following way.

2. If you know the morphology of the target bacteria, you can directly pick the miscellaneous bacteria for plate culture until they grow into colonies and there are no miscellaneous bacteria under the microscope. Or selecting bacteria, diluting, and coating to obtain colonies.