High school biology PCR technique requires conditions of high temperature and what?

Requirements:1. Double-stranded DNA template

2. Heat-stable DNA polymerase (Taq enzyme) (first discovered by Taiwanese scientist Chia-Yun Chien in Thermus thermophilus at Yellowstone Park, USA)

3. Primer (a small piece of DNA or RNA with a specific sequence)

4. Four types of deoxyribonucleotides

5. Unwinding ( Denaturation) temperature:90~95'C

Primer binding (denaturation) temperature:55~60'C

Synthesis (elongation) temperature:70~75'C