Brief introduction of teratogenic test

Refers to the standard test to identify the teratogenic effect of foreign compounds by using experimental animals. Teratogenesis test can detect the toxic effects of test subjects, such as embryo death, structural malformation and growth retardation. Rats or mice are often used to mate with sexually mature experimental animals. Pregnant rats are randomly divided into two groups, and the sperm found on vaginal suppository or smear is the 0-day pregnancy. There are usually three dose groups and 1 control group, with 20 pregnant rats in each group. The high dose group should cause obvious toxic reaction to the female rats, but the mortality rate of the female rats should not exceed10%; There should be no obvious toxic reaction in the low dose group. The subjects were in the organogenesis stage of embryonic development (6- 15 days for rats and 5- 14 days for mice). Before delivery 1-2 days, the intrauterine tube was removed by laparotomy, and the number of live fetus, stillbirth and absorption was recorded, and the appearance, skeleton and visceral malformation of live fetus were examined. In the treatment group, the incidence of maternal fetal malformation was calculated by the number of maternal fetuses, and the fetal malformation rate and single malformation rate were calculated by the number of fetuses, and compared with the control group.