Is it induced in vivo or cultured in vitro to produce monoclonal antibodies on a large scale?

There are two main methods for mass production of monoclonal antibodies:

(1) A large number of hybridoma cells were cultured in vitro by rotating culture tubes, and monoclonal antibodies were obtained from the supernatant. However, the yield of this method is low, and the antibody content in the culture medium is generally 10 ~ 60μ g/ml. If mass production, the cost will be high.

(2) Inoculating hybridoma cells in vivo to prepare ascites or serum.

① Solid tumor method: Inoculate hybridoma cells in logarithmic growth period at the ratio of1~ 3x107/ml under the back of mice, and inject 0.2 ml at each place, with * * * 2 ~ 4 points. After the tumor reaches a certain size (generally 10 ~ 20 days), blood can be collected and monoclonal antibodies can be obtained from serum.

The antibody content can reach 1 ~ 10 mg/ml. But the amount of blood collected is limited.

② Ascites preparation: routine intraperitoneal injection of 0.5ml.

Basal shark alkane

BALB/C mice were injected with 1× 106 hybridoma cells intraperitoneally after 0/~ 2 weeks, and ascites was produced after 7 ~ 10 days of inoculation.