Isolation and purification of microorganisms selected selective culture methods and techniques, what is the impact on the results of the experiment

Selective culture methods and techniques chosen for isolation and purification of microorganisms, and how they affect the experimental results

Factors affecting the isolation of pure lines of microorganisms:

1. Infringement by other bacteria;

2. Mutations in the process of growth;

3. Temperature and humidity must be appropriate.

Introduction:

Pure line isolation of microorganisms: a method of selection in which some method is used to try to pick out certain cells to the level of "colony line" or "strain line".

Methods of pure lineage isolation of microorganisms:

(1)Dilution plate isolation

The samples to be examined will be diluted step by step according to a certain method to a certain concentration gradient, select the samples of the appropriate concentration of the dilution and inoculate them, and incubate them for a certain period of time, and then the microbial colonies can be obtained.

(2) Plate streaking

The inoculation tool is used to pick the appropriate amount of samples (microbial mixture), according to a certain method of streaking in the medium plate, after a certain period of time of cultivation, microorganisms can be obtained colonies, and then obtain the microorganisms of pure cultures.

(3) Selective culture isolation

According to the unique physiological characteristics of the cultured microorganisms, the use of selective media for culture is highly targeted.