Traditional Culture Encyclopedia - Traditional stories - Briefly describe the methods, advantages and disadvantages of bacterial detection.
Briefly describe the methods, advantages and disadvantages of bacterial detection.
You can directly observe its movement with a microscope. In addition, flagella is the motor organ of bacteria, and the existence of flagella can indirectly judge whether bacteria have motive. The method in memory is as follows:
1. Use a microscope
The activity of bacteria in bacterial liquid can be observed by some special methods with ordinary optical microscope. If possible, you can also use some high-end microscopes, such as phase contrast microscopes.
2. Use semi-solid medium for culture.
Flagellar bacteria can spread and grow along the puncture line, and the periphery of the puncture line will become blurred; Bacteria without flagella can only grow along the puncture line, and the surroundings are clear and transparent.
3. Flagella staining
Dyed with special dyes for easy observation under a microscope.
4. Immunological methods
The existence of bacterial flagella was detected by antigen-antibody reaction.
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