Brief introduction of polymerase chain reaction

Polymerase chain reaction (PCR), also known as "free bacteria" cloning technology, is a new method for rapid amplification of specific DNA fragments in vitro. 1985, Muhlis of the human genetics laboratory of PE- Cetus Company in the United States invented the epoch-making polymerase chain reaction. This method changes the mode of traditional molecular cloning technology, does not interact with living cells and is simple to operate. Within a few hours, several copies of the template sequence and even a DNA molecule can be amplified by 10 7 ~ 10 8 times, which greatly improves the yield of DNA. It has been widely used in various fields of molecular biology research.