How many staining methods are there after two-dimensional electrophoresis? EB, trial dyeing, ECL dyeing and so on. Can you elaborate on their advantages and disadvantages? Online, etc

Because of the different sensitivities of various dyeing methods and the different specificities of different types of protein, each dyeing method has its advantages and disadvantages. The sensitivity of silver staining or fluorescent staining is higher than that of inspection staining: moreover, fluorescent staining can not display nucleic acid, and the background (high signal-to-noise ratio) is low in matching with mass spectrometry, so it needs to be equipped with an imaging instrument that can detect it, which is very popular in some large laboratories; Silver staining method is widely used, and glycoprotein which is difficult to detect can be detected. Examination and dyeing are traditional and relatively cheap, which are commonly used in laboratories. If strictly analyzed, dyeing can be combined in two or more ways.