What are the common detection methods for bacteria in pathogenic microorganisms

1, rapid test piece technology method

Rapid test piece refers to the paper, paper film, film, etc. as a medium carrier, the specific medium and coloring substances attached to it, through the growth of microorganisms in the above, color to determine the microbial method of food.

The development of the total bacterial count detection paper began in the 1980s, and its main advantages are simple, practical, economical and highly operational. In recent years, the test piece with filter paper and Petrifilm of a U.S. company as the carrier has begun to be widely used.

2. Bioelectrochemical methods

Bioelectrochemical methods refer to the method of determining the electric charge produced or consumed by microorganisms through electrodes, thus providing analytical signals. Microorganisms in the process of breeding and metabolism, the electrochemical properties of the culture medium such as current, potential, resistance and conductivity will change, so it can be detected and analyzed by the changes in these electrochemical parameters to achieve rapid determination of microorganisms.

The common ones are: impedance analysis, potential analysis, current analysis and so on. Bioelectrochemical methods are characterized by rapid measurement, intuitive, simple operation, low cost of measurement equipment and controllability of the signal.

3, microcolony technology

Microcolony refers to the early growth and reproduction of bacteria in the solid-phase carrier formed only with the help of microscope observation of tiny colonies. Microcolony technology has a rapid, economical and practical characteristics, its research began in the 1950s, quantitative determination of technology from the 1970s, foreign reports have been reported that the method is applied to the rapid detection of the total number of bacteria in water, food.

4, gas chromatography

Gas chromatography is applied to the detection of microorganisms, mainly based on the chemical composition of different microorganisms or their metabolites produced by different, the use of the above chromatographic detection can be directly analyzed in a variety of bodily fluids in the bacterial metabolites, fatty acids, proteins, amino acids, peptides, polysaccharides, etc., in the cell to determine the specific chemical markers of pathogenic microorganisms, to assist in the diagnosis and detection of pathogenic components. , assisting in pathogen diagnosis and detection.?

5, high performance liquid chromatography

The use of high performance liquid chromatography detection can be analyzed in a variety of body fluids, bacterial metabolites, pathogenic microorganisms, etc., in order to determine the specific chemical markers of pathogenic microorganisms, to assist in the diagnosis and detection of pathogens.

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