Simulated phosphorylated kinase has no kinase activity.

Does the subject want to ask, "what is the reason why the kinase after phosphorylation of simulated kinase has no kinase activity?" Because phosphorylation changes the conformation or structure of kinase, kinase cannot bind to its substrate or catalyze the reaction of substrate. In addition, the activity of kinase may also be affected by other factors, such as reaction conditions, pH value, ion concentration and so on.