Protein electrophoresis, why do you want boiling water bath before loading?

Only boiling can eliminate the stereoscopic secondary structure of protein and extend it into a one-dimensional linear structure, so generally speaking, the dimer will dissociate, and electrophoresis can be carried out completely according to the molecular weight. The added protein label has the significance of indicating the molecular weight! Dithiothreitol or mercaptoethanol can destroy disulfide bonds and accelerate protein denaturation. The existence of SDS can make each amino acid have the same charge and make the whole protein linear. 100 degree boiling 100 minute, centrifuge for 5 minutes, and take the supernatant for electrophoresis, because precipitation will lead to tailing. You can also take the supernatant to another test tube and prepare it again after a week at 4 degrees.