Try to describe the operation method of label separation.

Please describe the operation method of tag separation as follows:

Scribing separation is a common isolation and purification of microorganisms method, and its operation steps are as follows:

Preparation of tool materials: the streaking separation needs to be carried out in a sterile environment, and tools such as sterile inoculation rings, needles, alcohol lamps, sterile Petri dishes, sterile cotton swabs, sterile bacteria culture medium and strains to be separated need to be prepared.

Ignition of alcohol lamp: Before the operation, it is necessary to ignite the alcohol lamp to provide the heat needed for aseptic environment.

Line inoculation: dip the strain to be separated with a sterile cotton swab, and then gently press it on the surface of the culture medium of a sterile Petri dish to make the strain adhere to the culture medium. Then the strain is streaked or inoculated on the culture medium with an inoculation ring or needle, and inoculation is usually carried out by streaking with different shapes such as "+"or "++".

Culture observation: the inoculated Petri dish is cultured in a constant temperature box, and it usually takes a period of time (such as 24-48 hours) to observe the growth of the strain.

Single colony selection: during the culture process, single colonies with different shapes and colors can be seen. Single colonies were selected with sterile inoculation rings or needles and inoculated into new Petri dishes.

Repeated streaking separation: For complex flora or strains that need further purification, it may be necessary to repeat the above steps for multiple streaking separation to obtain more pure strains.

Preserve the strain: Finally, preserve the purified strain in a suitable environment for subsequent research or application.

It should be noted that the operation of line separation requires strict aseptic operation skills and control of experimental conditions to ensure the acquisition of purified strains and avoid pollution. In addition, for different strains and experimental requirements, it may be necessary to make appropriate adjustments and optimize the operation steps.