Traditional Culture Encyclopedia - Traditional culture - What are the similarities and differences between page-sds electrophoresis and paper electrophoresis?
What are the similarities and differences between page-sds electrophoresis and paper electrophoresis?
Working principle: PAGE-SDS electrophoresis uses charge effect and molecular sieve effect for separation, while paper electrophoresis mainly uses charge effect for separation.
2. Resolution: In some cases, the resolution of PAGE-SDS electrophoresis is higher than that of paper electrophoresis. This is mainly because the upper limit of its high molecular weight can filter out most of the low-abundance protein, thus improving the resolution.
3. Steps and equipment: Paper electrophoresis is generally carried out on a "wet film" made of glass fiber, while the steps and equipment of PAGE-SDS electrophoresis are different from traditional electrophoresis and need to use a specific gel medium.
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