What are the general quantitative analysis methods in HPLC?

There are generally two types of gas chromatography quantitative detection, one is the external standard method and the other is the standard method. If there is no standard material, the volume area normalization method can only be used for rough quantification.

By detecting the content and emissions of various substances that have an impact on humans and the environment, we can track changes in environmental quality, determine the level of environmental quality, and provide a basis and guarantee for environmental management, pollution control and other work.

Simply put, understanding the environmental level and conducting environmental monitoring are the prerequisites for carrying out all environmental work.

Extended information: HPLC is divided into normal phase chromatography and reverse phase chromatography according to the components of the stationary phase and mobile phase.

Normal phase chromatography uses a polar stationary phase (such as polyethylene glycol, amino and nitrile bonded phases); the mobile phase is a relatively non-polar hydrophobic solvent (alkanes such as n-hexane, cyclohexane), usually

Add ethanol, isopropyl alcohol, tetrahydrofuran, chloroform, etc. to adjust the retention time of the components.

It is often used to separate moderately polar and highly polar compounds (such as phenols, amines, carbonyls, amino acids, etc.).