Comparing the advantages and disadvantages of tissue isolation method and spore isolation method

1. Advantages: applicable to many different species of microorganisms; effective for microorganisms that are not very resistant to heat or drying, microorganisms can be extracted directly from samples (e.g., soil, water samples, etc.).

2, disadvantages: need a longer time to process, because the need to complete the process of cultivation of the colony; the existence of selectivity, some bacteria can not be successfully isolated, will get mixed colonies, increasing the difficulty of identification.

Spore isolation method:

1. Advantages: spores have strong resistance and can survive in adverse environments; isolation of spores can more accurately determine the type of contamination of the sample, spore isolation is faster, usually only a few days can be separated and cultured to obtain a single pure bacteria.

2. Disadvantages: the method can only be used for the isolation of spore-forming bacteria; will miss some non-spore-forming microorganisms, need special conditions to isolate spores, including high temperature, low humidity and other conditions.