Traditional Culture Encyclopedia - Traditional culture - What is the quality evaluation system of traditional Chinese medicine polysaccharide?
What is the quality evaluation system of traditional Chinese medicine polysaccharide?
According to the characteristics and pharmacological effects of polysaccharides, the quality evaluation system of traditional Chinese medicine polysaccharides mainly includes the following evaluation indicators:
1. Polysaccharide content: To evaluate the total content of polysaccharide in the polysaccharide sample of traditional Chinese medicine. Generally, physical and chemical determination methods are used, such as phenol sulfuric acid method and red stone core method.
2. Molecular weight: To evaluate the molecular weight distribution range and average molecular weight of traditional Chinese medicine polysaccharide. Commonly used determination methods are chromatography and gel permeation chromatography.
3. Physical and chemical properties: Evaluate the physical and chemical properties of traditional Chinese medicine polysaccharides, such as solubility, relative density, viscosity, etc.
4. Configuration and structure: To evaluate the molecular configuration and structural characteristics of traditional Chinese medicine polysaccharides, such as chain length, monosaccharide composition, glycosyl connection mode, etc. Commonly used identification methods include nuclear magnetic resonance (NMR), infrared spectroscopy (IR) and mass spectrometry (MS).
5. Biological activity: To evaluate the biological activity and pharmacological activity of traditional Chinese medicine polysaccharide, such as antioxidation, antitumor and immunomodulation. Commonly used experimental methods include in vitro cell test and animal experiment.
The establishment of quality evaluation system of traditional Chinese medicine polysaccharide aims to ensure the stability and reliability of quality and curative effect of traditional Chinese medicine polysaccharide, and provide scientific basis for quality control and clinical application of traditional Chinese medicine. The selection of specific evaluation indexes and methods needs to be determined in combination with different varieties of Chinese medicine polysaccharides, as well as the research purpose and needs. Specific evaluation criteria and methods can refer to relevant pharmacopoeia, standards or research literature.
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