Brief introduction of single cell genome sequencing

Single cell whole genome sequencing technology is a new technology to amplify and sequence the whole genome at the single cell level. The principle is to amplify the isolated small amount of whole genome DNA of a single cell to obtain a complete genome with high coverage, and then use high-throughput sequencing to reveal the relationship between cell population differences and cell evolution. From the methodological point of view, obtaining high coverage and high fidelity whole genome amplification products is the guarantee of accurate and comprehensive sequencing results. Malbac (Multiple Annealing and Looping Based Amplification Cycles), invented by Academician Xie Xiaoliang of Harvard University, is the most advanced technology in the field of single cell amplification, which greatly improves the amplification coverage, uniformity and sensitivity compared with previous nonlinear or exponential amplification methods.