The monoclonal antibody purification methods with the best purification results are

1. Affinity Chromatography: Separation of antibodies using antibody specific recognition. Usually, adsorbents, such as protein A, protein G or protein L, are used to perform affinity chromatography against the common heavy and light chains, Fc region and other parts of the antibody.

2. Ion exchange chromatography: The interaction of surface charged residues in a molecule with an oppositely charged ion exchange matrix is utilized to specifically purify the target molecule. It can be selected according to the isoelectric point of the antibody.

3. Gel filtration chromatography: selective separation of antibodies and other molecules using pore size to remove macromolecular impurities.

4, reversed-phase chromatography chromatography: according to the different hydrophilicity of the target molecules and contaminant molecules in the solution, the use of electrostatic force to separate antibodies. Reversed-phase chromatography has advantages such as insensitivity to ionic strength, and is widely used in the purification process of antibodies.

The choice of monoclonal antibody purification method will be affected by many factors, such as the concentration of the mother liquor, cationic contamination, PH value and so on.