What is the principle of flow cytometry?

Principle: According to the intensity of excitation light, judge the combination of cells and staining substances, so as to get the results to be detected.

1. After staining the cells to be tested, prepare single cell suspension.

2. Press the sample to be tested into the flow chamber at a certain pressure, and at the same time, the phosphate buffer solution without cells is sprayed from the sheath fluid tube at a high pressure. The direction of the sheath fluid tube forms a certain angle with the flow direction of the sample to make the sheath fluid flow around the sample at a high speed to form a circular flow, and the cells to be tested are lined up under the sheath fluid and pass through the detection area in turn.

3. Flow cytometry usually uses laser as the light source, and the focused and shaped beam irradiates the sample flow vertically, and the cells dyed by fluorescence generate scattered light signals and fluorescence signals under the irradiation of laser beams.