Traditional Culture Encyclopedia - Traditional customs - What are the disadvantages of enzyme screening method based on gene sequence?
What are the disadvantages of enzyme screening method based on gene sequence?
Causing biosafety problems.
Polymerase chain reaction (PCR) is a method of enzymatic synthesis of specific DNA fragments in vitro, which consists of several reactions such as high-temperature denaturation, low-temperature annealing (renaturation) and appropriate temperature extension, and it is easy to cause safety problems.
Cycling can quickly amplify the target DNA, which has the characteristics of strong specificity, high sensitivity, simple operation and time saving.
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