What is the calculation formula of cell counting plate?

Cell number in cell suspension/ml = total number of 4 large cells /4× 10000. If it has been diluted before counting, it can be multiplied by the dilution multiple.

When counting, only complete cells are counted, and if cells gather together, it is counted as one cell. In a big square, if there are cells located on the line, the cells below the line are generally not counted, nor are the cells on the left line. The error of secondary repeated counting should not exceed 5%. Under the microscope, it is the living cells that are highly refractive and uncolored, and the dead cells that are dyed blue.

Extended data:

Precautions:

Be sure to disperse into a single cell, and thoroughly mix the cell suspension before sampling and counting.

When counting under a microscope, if you encounter a cell mass composed of more than 2 cells, it should be counted as a single cell. If the cell mass is >: 10%, the cell dispersion is insufficient.

Count the total number of cells in a square with four corners under a microscope. For the cells that press the line, only the top, bottom, left and right lines are counted, and they are counted as cell clusters as a single cell.

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