Molecular typing method of Brucella

Hello, what is the molecular typing method of Brucella? Molecular typing of Brucella is a method to distinguish and classify different strains by using gene sequence difference or fingerprint technology. According to different species and strains, different genes or sequence regions are amplified by PCR, and the amplified products are sequenced or analyzed by other methods to obtain different differences or fingerprints, so as to realize the identification and typing of strains. At present, the commonly used molecular typing methods of Brucella are multiplex PCR typing, multiplex gene sequencing typing, multiplex fingerprint analysis, whole genome sequencing and so on. Multiplex PCR typing mainly aims at different PCR product sizes, and obtains different bands through electrophoresis analysis, thus distinguishing different strains. Multi-gene sequencing typing is to sequence specific genes, analyze the similarities and differences of sequences through machine learning and other algorithms, and realize the classification and identification of strains. Multiple fingerprint analysis is to obtain specific bands by using different DNA fingerprinting techniques, so as to realize the classification and identification of strains. In a word, Brucella molecular typing is an accurate, rapid and reliable identification and typing method commonly used at present, which has been widely used and popularized in epidemic tracking, clinical diagnosis, epidemiological research, prevention and control, etc.