Traditional Culture Encyclopedia - Traditional festivals - Briefly describe the preparation scheme, process application and advantages of monoclonal antibodies! (Urgent, Urgent, Urgent!)

Briefly describe the preparation scheme, process application and advantages of monoclonal antibodies! (Urgent, Urgent, Urgent!)

1. Injection of antigen into an organism to cause an immune response.

2. Isolation of immunized effector B cells from blood.

3. culturing myeloma cells.

4. Fusion of myeloma cells with effector B lymphocytes to obtain hybridized tumor cells.

5. Screening and culturing the hybridoma cells to produce monoclonal antibodies.

Advantages: high purity, high specificity, accurate, efficient, simple and fast in disease diagnosis.