How to separate protein from starch?

Leaching method is also called soaking method. In order to extract substances from solid mixtures or organisms, the extractant used should be able to dissolve the extracted substances in large quantities without destroying their properties.

2, solvent extraction method:

Solvent extraction is used to extract a component from a solution. By using the difference of partition coefficients of components in two immiscible reagents, the components are transferred from one solution to another solvent, so as to be separated from other components and achieve the purpose of separation and enrichment.

3. salting-out method

Adding some inorganic salts to the solution will greatly reduce the solubility of solute in the original solvent, but it will precipitate out of the solution. This method is called salting out. For example, a large amount of salt (ammonium sulfate), especially heavy metal salt, is added to protein solution to precipitate protein from the solution.

4. Extraction method

Extraction is a unit operation to separate mixtures by using the principle of similar miscibility. According to the different components extracted, it can be divided into extraction method (solid-liquid extraction method) and extraction method (liquid-liquid extraction method). Extraction method (liquid-liquid extraction method): separation is realized by using different distribution coefficients of extracted components in two immiscible solvents.

5. decompression concentration method?

Some components to be tested are unstable to heat and easy to decompose at higher temperatures. Reducing the boiling point of the solvent by vacuum concentration can not only quickly concentrate to the required volume, but also avoid the decomposition of the detected substance.

The common vacuum concentration equipment is all-glass vacuum concentrator, also known as K-D concentrator. This instrument is a common vacuum distillation device. This method has the characteristics of low concentration temperature, high speed, low loss and easy control of required volume. It is suitable for the concentration of the extract of the thermally unstable test object, especially for the concentration of the sample solution in the analysis of pesticide residues. In addition, it can also be used for purifying and distilling solvents.