Traditional Culture Encyclopedia - Traditional festivals - Microscope has optical, electronic, ultraviolet and other types, what is the difference between them?
Microscope has optical, electronic, ultraviolet and other types, what is the difference between them?
Objective according to the different conditions of use can be divided into dry objective and immersion objective; which immersion objective can be divided into water-immersion objective and oil-immersion objective (commonly used magnification of 90-100 times).
These different uses of the microscope can be targeted for magnification, so for more areas with better help. The role of microscopes allows humans to see the world more clearly, which is a great blessing for mankind.
The metallurgical microscope is the traditional optical microscope and the computer through the photoelectric conversion organic combination, not only in the eyepiece for microscopic observation, but also in the computer display screen to observe the real-time dynamic image, computerized metallurgical microscope and can be edited, save and print the required picture.
The sample is scanned with a finely focused electron beam, so the sample must be able to withstand a high vacuum and must be reasonably conductive. (This is because you are stacking a stream of electrons into the sample and must conduct the current away.) SEM samples are usually coated with a very thin coating of carbon or metal (such as gold or chromium) to make them conductive.
Optical microscopes usually use visible light with long wavelengths as a light source, while electron microscopes use electron beams with very short wavelengths as a transmission source.
The difference between the two light sources dictates that they cannot be used together in combination.
Additionally, optical microscopes are complex precision instruments that require stable high-voltage systems, vacuum systems, imaging systems, and special sample preparation methods, which are far more demanding than ordinary optical microscopes.
Then a class of samples is obviously limited to transparent samples, which is also the most typical example of biomolecular samples, because the cells, including many organelles appear to be transparent, and some samples can not be stained, which makes the field of view a bright and can not distinguish between the samples. To solve the problem, optical microscopy, you can use dark-field microscopy, phase contrast microscopy and differential interference phase contrast microscopy (DIC) to solve; higher resolution requirements, you need to use electron microscopy.
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