Traditional Culture Encyclopedia - Traditional festivals - Advantages and disadvantages of different analytical methods such as HPLC-EL-SD, hplc-uv and colorimetry.
Advantages and disadvantages of different analytical methods such as HPLC-EL-SD, hplc-uv and colorimetry.
1. Advantages: HPLC-EL-SD is an important separation and analysis method, which has been widely used; Hplc-uv plays an extremely important role. It is estimated that about 7% of the drug standards are determined by hplc-uv. Colorimetric method was determined by spectrophotometry and titration after the colored eluent was collected in the column in the early stage.
2. Disadvantages: the particle size of the stationary phase used in HPLC-EL-SD column is very small (below 1μm), so its separation efficiency is low; The mobile phase in hplc-uv can choose liquids with different polarities, which lacks affinity. Colorimetry is applied to the separation and analysis of compounds with high boiling point, poor thermal stability and large molar mass, and is limited to the separation and analysis of compounds and gases with low boiling point.
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