Microbial culture method

Microorganisms cultivated by microbial culture are usually germs, bacteria, actinomycetes, fungi and so on. It belongs to a kind of biological culture.

Microbial culture steps:

1. Prepare nutrient solution dilutions with different concentrations: dissolve 1 ml nutrient solution in 9 ml sterile water for 5 minutes, and shake well for 5 minutes to prepare 10% solution.

2. Suck 1 ml from the solution near the mouth of the container with a straw (be careful not to face it) and add it to a test tube containing 9 ml of sterile water. By analogy, different concentrations of diluents such as 1%, 0. 1% and 0.0 1% will be made.

3. Put the mouth of the test tube close to (be careful not to face) the flame of the alcohol lamp, hold the test tube in your left hand and the Petri dish in your right hand (only a small crack is exposed, close to the flame). Pour the solution into Petri dishes and mark the concentration on each dish.

4. Place the mouth of the sample container with bacteria growth close to (be careful not to face) the flame of alcohol lamp, pick up a small amount of bacteria on the colony with inoculation ring, and add it to 10% nutrient solution. Put the inoculation ring on an alcohol lamp for burning and sterilization, then take another sample and add it to a Petri dish filled with 1% nutrient solution (only a small slit is exposed, close to the flame). Repeat this operation and add the two.

5. Shake the inoculated Petri dish gently and keep it in a thermostat at 37 degrees Celsius. After 3 days, colonies will appear.